Immunochemical Methods for Quantitation of Vitamin B6
WESTERN REGIONAL RESEARCH CENTER BERKELEY CA
Pagination or Media Count:
A procedure is described which proposes schemes for determining the total of all B6 vitamins in acid-hydrolyzed samples utilizing a radio- immunoassay RIA or an enzyme-immunoassay EIA. Sample preparation is similar for both RIA and EIA. Two specific antibodies antipyridoxine and antipyridoxamine are employed to determine pyridoxamine, a portion of the sample is reduced with sodium borohydride. Pyridoxal is determined by difference between pyridoxine before and after reduction. The results indicate that two procedures have been developed which are selective for pyridoxamine the fluorescent enzyme immunoassay and the spin immunoassay and one assay which is equally sensitive to pyridoxine and pyridoxamine the radio-immunoassay.
- Anatomy and Physiology