Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.
Annual progress rept. no. 1, 1 Jul 79-1 Jan 80,
YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE
Pagination or Media Count:
An in vitro assay was developed to detect transmission of Dengue-2 parent and vaccine virus strains by vector mosquitoes. After interthoracic inoculations, transmission of both viruses was detected at 7 and 14 days incubation. Rates of transmission were similar. A mosquito inoculation-headsquash-immunofluorescence technique was found to be more sensitive than Aedes albopictus cells C636 for the isolation of dengue virus in the in vitro transmission assay. Flavivirus viremias in suckling mice proved relatively noninfectious for Aedes aegypti. LLC-MK2 cells were adapted to a medium containing calf serum in order to conserve fetal calf serum. Cells were maintained and grown in calf serum fetal calf serum was used only in the overlay medium used in the plague assay test.
- Medicine and Medical Research