Accession Number:

ADA129721

Title:

Platelet-Endothelial Function in Relation to Environmental Temperature,

Descriptive Note:

Corporate Author:

ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA

Report Date:

1983-06-06

Pagination or Media Count:

27.0

Abstract:

Thromboembolic phenomena are commonly observed in frostbite injury to the microvasculature. Large blood vessel endothelium was used to assess any alterations in platelet-endothelial interaction resulting from low environmental temperature. Media from bovine endothelial cells 37, 24, 4 C did not cause or enhance platelet aggregation. Yet ADP 120 micrometers stimulated aggregation of all platelet samples. Bovine and procine endothelial cells were incubated at 37, 24, 4, and 0.5 C with homologous platelets suspended in tyrodes buffer plus albumin, and the rate of ADP-stimulated platelet aggregation was compared with control platelets. Bovine venous endothelial cells at 37 C inhibited platelet aggregation more strongly that aortic endothelial cells. This inhibition was blocked by aspirin 7 mM and was independent of environmental temperature. Porcine endothelial cells also inhibited platelet aggregation. This inhibition maximal at 37 C was significantly attenuated by temperatures or 24 C. A significant loss of inhibition of platelet aggregation could lower the threshold at which parenchymally-derived nucleotides of other substances from damaged cells can cause aggregation. Thus, in porcine endothelial cells bovine endothelial cells, a possible mechanism for the thromboembolic action of cold is suggested. Author

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE