Morphogenesis of Dengue Virus: Molecular Biology and Molecular Organization of Proteins.
Final rept. Nov 71-Jul 80,
CALIFORNIA UNIV DAVIS DEPT OF BACTERIOLOGY
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The first major finding was the appearance of Dengue antigens on the surface of infected cells. This was of major importance since it has been hypothesized that dengue hemorraghic fever DHF is the result of DEN antigen interaction with anti Dengue antibody resulting complement mediated vascular endothelial injury. Findings then led us to postulate that Dengue antigens on the cell surface were composed of ordered arrays of Dengue polypeptides. As a first approach we proposed to examine the surface of the virus itself and then proceed to a study of the cell surface. To do this we studied the growth of Dengue in BHK cells. Our findings established new growth conditions and assay procedures for the virus. Since our approach was to use crosslinking reagents to study the organization of the Dengue polypeptides we examined the charge properties of viral glycoproteins on two other togaviruses. Results of these studies showed that the glycoproteins of two closely related viruses were quite different but that these differences probably would not significantly alter the organization of polypeptides on the surface of the virion. Protein-protein interactions on Japanese encephalitis virus JEV, a virus closely related to Dengue, were analyzed. These results demonstrated that the major viral glycoprotein on the surface of the virion had as its nearest neighbor either one of its kind or another newly defined viral glycoprotein. Thus the clustering of viral antigens on flaviviruses had been demonstrated.
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