Bioconcentration, Elimination and Metabolism of Picric and Picramic Acid in Freshwater Fish and Estuarine Bivalves.
Final rept. 29 Apr 82-30 Apr 83,
JOHNS HOPKINS UNIV LAUREL MD APPLIED PHYSICS LAB
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The bioconcentration, elimination and metabolism of 14C-picric acid and 14C-picramic acid were evaluated for rainbow trout Salmo gairdneri and American oysters crassostrea virginica continuously exposed to the compounds for 42 days followed by elimination for 14 days in clean waters. Acute toxicity was also determined for both compounds and species. Measurements of the uptake of both picric and picramic acid by rainbow trout showed that both compounds do not accumulate in the epaxial muscle tissues at levels above the mean exposure concentration in the water column. American oysters, however, do bioconcentrate both picric and picramic acid andor their metabolites at significantly higher levels than exposure concentration levels. The uptake rate of both picric and picramic acid was biphasic. A well defined steady state was not reached during the 42-day exposure period for the compounds. The 42-day bioconcentration factor BCF for exposure to 0.45 mgL picric acid was 65.5, while the 42-day BCF for exposure to 0.05 mgL picric acid was 16.5. The 42-day BCFs for exposure to 0.24 and 0.02 mgL picramic acid were 49.3 and 86.6, respectively. No statistically significant elimination of picric acid was observed for oysters during the 14-day elimination day study. Depuration of picramic acid from oysters was biphasic.