Effect of Nonadecafluorodecanoic Acid on Microsomal Stearoyl-CoA desaturase and Electron Transport Activities in Rat Liver
Technical rept. 1 Jun-31 Jul 1982
SOUTHEASTERN CENTER FOR ELECTRICAL ENGINEERING EDUCATION INC ST CLOUD FL
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The biochemical basis of the hepatotoxicity of NDFDA nonadecafluorodecanoic acid has been investigated from the point of view of its effects on stearoyl-CoA desaturase and associated electron transport functions in microsomes. Changes in these parameters would be consistent with earlier observations on altered liver fatty acid content in NDFDA-treated rats. In these preliminary studies, hepatic stearoyl-CoA desaturase activity dropped in both NDFDA-treated rats and in pair-fed controls, approaching zero within 6-8 days. This decrease was attributed mainly to decreased food intake and did not appear to be directly related to NDFDA hepatotoxicity. However, NDFDA induced several biochemical changes not present in the pair-fed controls. These included 1 decreased inducibility of stearoyl-CoA desaturase by force feeding an amino acidsucrose mixture, 2 a marked decrease in the rate of microsomal electron transport from NADH through cytochrome b5 to the terminal oxidases including the desaturase and molecular oxygen, and 3 an increase in the concentration of cytochrome P-450, an important component of the microsomal drug methobolizing system.
- Anatomy and Physiology