Effect of Hydrazines on Substrate Utilization by a Strain of Enterobacter Cloacae
AIR FORCE AEROSPACE MEDICAL RESEARCH LAB WRIGHT-PATTERSON AFB OH
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Hydrazine and several of its methylated derivatives have been the subject of extensive investigations for nearly eight decades. The application of short-term in vitro toxicity test systems to the study of xenobiotic molecules suggested consideration of the response of bacterial systems to hydrazines. London showed the toxicity of hydrazine Hz, monomethylhydrazine MMH, and 1,1-dimethylhydrazine UDMH to a strain of soil bacteria to be comparable to that observed in other biological systems. A subsequent study by Mantel London suggested different mechanisms of toxicity for Hz and MMH as indicated by growth rates of cultures grown previously in the presence of one of the compounds. During a series of preliminary experiments on the death rate kinetics of Hz exposed cultures, we noted growth in substrate-free media that suggested metabolites derived from lysed cells were being metabolized. This observation in conjunction with reports on the effects of Hz intoxication on carbohydrate metabolism prompted an investigation of the effects of Hz, MMH, and UDMH on the utilization of arbitrarily selected saccharides and compounds involved in intermediary metabolism as a means of elucidating mechanism of action.