The Mechanism of Binding and Secretion of Alkaline Phosphatase Across the Cell Membranes of Pseudomonas aeruginosa.
DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)
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During growth, pH of the medium decreases resulting in the inactivation of cell-free and surface-bound alkaline phosphatase, whereas periplasm-located alkaline phosphatase is not affected. The decreased pH of the medium induced permeability changes in the outer cell wall which resulted in the complete release of alkaline phosphatase after sucrose extraction. The permeability change of the outer cell wall is pH dependent and reversible. The periplasm-located alkaline phosphatase remains constant during growth of Pseudomonas aeruginosa. A hypothetical model of the mechanism for the release of periplasm-located proteins into the growth medium during growth is presented. Author