Signal-to-Noise Considerations in Fluctuation Analysis Spectroscopic Techniques (FAST).
Interim technical rept.,
INDIANA UNIV AT BLOOMINGTON DEPT OF CHEMISTRY
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It is possible to determine fluorescence lifetimes from the frequency response of a fluorophore as well as from its time response. Various schemes for measuring such a response curve are illustrated and include the use of sources whose intensity is modulated at single, swept frequencies, at several frequencies simultaneously, or over a broad, continuous band of frequencies. Detection methods which are considered include a single broad-frequency-response device, one which is swept, and another which hops from one modulated frequency to another. Signal-to-noise expressions are derived for each combination of source and detector and conclusions are drawn from comparison of the SN expressions. From these considerations and the availability of existing sources for use in time-resolved fluorimetry, the most attractive approaches appear to be one which employs a source modulated at several frequencies simultaneously, each of which is monitored by its own tuned detector or a sinusoidally modulated source, swept synchronously with a tuned detector.
- Atomic and Molecular Physics and Spectroscopy