Improved Quantification of Plasma Catecholamines by the Radioenzymic Kit Method.
Final rept. Oct 80-Jun 82,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX
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A departure from a radioenzymic procedure widely used for plasma catecholamine analyses is reported. The departure features use of mean net standards instead of individual internal standards. Interlaboratoryintermethod analyses validated use of the modified principle and demonstrated its preferential use over the conventional principle. Also demonstrated is use of the modified procedure for calculating catecholamine concentrations of several plasma samples collected from an exercising man and an acceleration-stressed swine. Benefits already derived from use of the procedure include the following a quantification of certain plasma samples which could not be satisfactorily quantified by conventional options, b an apparent gain in analytical precision in virtually all other analyses, and c a gain in economy of approximately 25.
- Test Facilities, Equipment and Methods