Effect of Diet and Pneumococcal Infection on Protein Dynamics of Blood Lymphocytes in Cynomolgus Monkeys.
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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Blood lymphocytes play an important role in host defense against infectious disease. To determine the effect of diet and infection on lymphocyte protein dynamics, cynomolgus monkeys received daily i.v. infusion of either 8 dextrose 34 kcalkg, 4.25 amino acids 16 kcalkg or AA D solutions. On day 2, 6 cynomolgus monkeys in each group received either live infected, I or heat-killed control S. pneumoniae. All cynomolgus monkeys were given a constant infusion of 14C leucine. By 6 h, the specific activity SA of the leucine in the protein-free filtrate of plasma had reached a plateau. A blood sample was then removed and the SA was determined on isolated leucine. I increased the rate of turnover and breakdown of total body protein. 8 dextrose significantly reduced leucine turnover and release in the breakdown of total body protein compared to 4.5 amino acids or amino acids dextrose. When infused with amino acids, the rate of protein synthesis of leucine was significantly increased compared to dextrose alone. Author
- Medicine and Medical Research