A Table for Rapid Determination of LOG10 LD50 or ID50 Values
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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While the determination of an LD50 or ID50 is neither a complicated nor a difficult procedure 1, many laboratories continue to perform these calculations on each titration manually. In some of our laboratory areas where access to a computer is not readily available, the table Table 1 presented below has been of great value in saving time, reducing errors, and enabling even untrained personnel to record LD50 values from titration results. Values shown are for titrations utilizing six animals per point, a commonly used number for titrating viruses or sera for antibody 2. The method of Reed and Muench 1 utilizes, at least in part, the large, total number of animals involved. The effect is that of using, at the two critical dilutions between which the endpoint lies, larger groups of animals than were actually included at these dilutions.
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