Development of a Vaccine against the Lethal Exotoxin of Pseudomonas Aeruginosa.
OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS
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The purpose of this project has been to produce non-toxic immunogenice fragments of exotoxin A from Pseudomonas aeruginosa for use in a vaccine. Initially, three phases of the project were identified. Phase I involved screening proteolytic agents for their ability to fragment the toxin and identifying non-toxic fragments. Phase II involved scaling-up the operation to produce larger quantities of non-toxic fragments and immunization of animals. Phase III deals with evaluation of immunization against the non-toxic fragments of the toxin using a burned mouse model. To date, we have been successful in fragmenting the toxin with several agents under a variety of conditions. However, we have not been able to show that any of the fragments are immunigenic, although they clearly are not toxic. The lack of immunogenicity may be due to the treatments used in generating the fragments. Thus, we have been concentrating our efforts on finding new methods of fragmenting and purifying potentially useful fragments.