Rapid Identification of Dengue Virus Serotypes Using Monoclonal Antibodies in an Indirect Immunofluorescence Test.
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
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Dengue and dengue hemorrhagic fever occur in epidemic and endemic form throughout tropical areas of the world. Dengue virus types 1, 2, and 3 have been associated with epidemics of major impact in the Western Hemisphere since the 1960s and in 1981 dengue type 4 was identified in the Carribean for the first time. Extensive serological crossreactions occurring among dengue virus serotypes as well as with other flaviviruses in commonly employed serological tests i.e., hemagglutination-inhibition, immunofluorescence, and complement fixation frequently interfere with identification of the antigenic type of dengue virus present in epidemic or endemic areas. Presently, the only certain method of identification is through the use of rigidly standardized reference antiserum in a virus plaque-reduction neutralization assay. Few laboratories possess sufficent resources to perform this test with the slowly replicating dengue viruses. Monoclonal antibodies that were produced using the recently established hybridoma technology have been used successfully to characterize viral antigens. The present study had as its objective the development of highly specific monoclonal antibodies suitable for rapid serotype identification of low passaged or unpassaged dengue virus for humans or insects using an indirect immunofluorescence test.
- Medicine and Medical Research