Determination of Nucleotides by Liquid Chromatograpy with a Phosphorus Sensitive Inductively Coupled Plasma Detector.
ARIZONA UNIV TUCSON DEPT OF CHEMISTRY
Pagination or Media Count:
Quantitative analysis of 100 microliters nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma ICP system. A single calibration using Na2HPO4 aq is useful for all nucleotides. A detection limit of 750 ng of phosphorus is determined with an RSD of 4.5. High performance liquid chromatography HPLC is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP is used as a selective detector by observing PI emissions at 213.6 nm. A Babington nebulizer which is used to aspirate the high percentage salt solutions with 6 efficiency is described. Author
- Atomic and Molecular Physics and Spectroscopy