Cryogenic Preservation of Granulocytes and Monocytes.
Final rept. 1 Dec 79-30 Nov 81,
CENTER FOR BLOOD RESEARCH BOSTON MA
Pagination or Media Count:
The feasibility of long term cryopreservation of granulocytes and monocytes has been established. Granulocytes of lower animals guinea pig, dog may be preserved for one to two years with small losses of cells and functionality. A species difference in stability exists in which baboon and human cells are the least stable. Human monocytes may be isolated in large 1 x 10 to the 9th power numbers as by products cells of plateletpheresis. They are stable and functional after months in liquid nitrogen. A protocol for granulocyte freezing containing a combination of extracellular HES and intracellular DMSO cryoprotectants is applicable with good results to all white cell types in animal blood including B and T lymphocytes.
- Medicine and Medical Research