Accession Number:

ADA109915

Title:

Partial Characterization of Small Plasmid Deoxyribonucleic Acid Present in ' Neisseria Meningitidis',

Descriptive Note:

Corporate Author:

DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)

Report Date:

1981-05-01

Pagination or Media Count:

18.0

Abstract:

The presence of plasmid DNA in Neisseria meningitdidis was investigated. N. meningitidis isolates from clinical meningitis and highly virulent in mice, were found to harbour a small plasmid. Agarose gel electrophoresis of crude lysates from different strains revealed a single plasmid DNA species with the same apparent electrophoretic mobility. Using agarose gel electrophoresis its molecular weight was calculated as 3.4 megadaltons. Electron microscopic observation revealed tdhat plasmid DNA molecules were predominently in the covalently closed form. The estimated contour length of the open circular form was 1.6 micro. The function of the plasmid DNA is presently unknown. Author

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE