Accession Number:

ADA109914

Title:

A Turbido-Haemolytic Assay for Complement,

Descriptive Note:

Corporate Author:

DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)

Personal Author(s):

Report Date:

1981-02-01

Pagination or Media Count:

22.0

Abstract:

A method for the haemolytic assay of complement is described. The method is based on the turbidimetric quantitation of sheep red blood cells at a wavelength of 640 nm. This approach allows for the continuous and simultaneous assessment of complement activity and results in a substantial reduction in the time required to perform complement assays as opposed to the conventional haemolytic assays. The turbido-haemolytic assay is used to investigate the anti-complementary activity of kappa and lambda carrageenans. Lambda carrageenan, the more sulphated polymer, is the more potent inhibitor of complement activation. The application of this technique to the detection andor identification of microorganisms is presently being investigated. Author

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE