Monitoring Calcium in Trout Eggs Exposed to Hydrazine.
Final rept. 1 Feb-1 Aug 81,
GRAMBLING STATE UNIV LA
Pagination or Media Count:
This study was designed to determine if certain hydrazine-induced morphological abnormalities could be attributed to reduced levels of available calcium. Rainbow trout eggs Salmo gairdneri were exposed to 48 hours to 1.0, 2.0, 4.0, 6.0, and 8.0 mgl of hydrazine. The responses to these concentrations were dose related. Eggs exposed to low concentration were less affected than those exposed to high concentration. Utilizing several staining techniques in combination with SEM, TEM and microelemental analysis, control muscle tissue showed a higher calcium level than healthy muscle of the 8.0 mgl hydrazine treated group. Maldeveloped muscle of the 8.0 mgl group showed a higher level of calcium than healthy muscle of the same individual. The notochord of the 8.0 mgl group showed a higher calcium level than the control group. The chorion did not reveal a calcium level although structural modifications occurred in the treated groups which resulted in a softening. Author
- Anatomy and Physiology