Accession Number:
ADA101133
Title:
A New Instrument for Time-Resolved Reduction of Scattered Radiation in Fluorescence Measurements.
Descriptive Note:
Interim technical rept.,
Corporate Author:
INDIANA UNIV AT BLOOMINGTON DEPT OF CHEMISTRY
Personal Author(s):
Report Date:
1981-07-01
Pagination or Media Count:
20.0
Abstract:
An opto-electronic cross-correlation system was employed to reduce the scattering influence in fluorescence measurements. A stable optical delay line incorporated into the instrument was positioned to yield detection at a fixed time after excitation the optimal delay time was determined simply from the ratio of the fluorescence decay curve to a similar curve portraying scattering response. Signal-to-scattering background enhancements greater than two were measured for the very short-lived TAU-0.07 ns fluorophores whereas a six-fold increase was measured for longer lifetime fluorophores. The shortest lifetime which would benefit from time-resolution in this system is limited by the time-response of the PMT 1.1 ns FWHM the excitation pulses are on the order of 6 ps. Author
Descriptors:
Subject Categories:
- Atomic and Molecular Physics and Spectroscopy
- Optics