A New Instrument for Time-Resolved Reduction of Scattered Radiation in Fluorescence Measurements.
Interim technical rept.,
INDIANA UNIV AT BLOOMINGTON DEPT OF CHEMISTRY
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An opto-electronic cross-correlation system was employed to reduce the scattering influence in fluorescence measurements. A stable optical delay line incorporated into the instrument was positioned to yield detection at a fixed time after excitation the optimal delay time was determined simply from the ratio of the fluorescence decay curve to a similar curve portraying scattering response. Signal-to-scattering background enhancements greater than two were measured for the very short-lived TAU-0.07 ns fluorophores whereas a six-fold increase was measured for longer lifetime fluorophores. The shortest lifetime which would benefit from time-resolution in this system is limited by the time-response of the PMT 1.1 ns FWHM the excitation pulses are on the order of 6 ps. Author
- Atomic and Molecular Physics and Spectroscopy