Accession Number:

ADA094352

Title:

Changes in Tissue Cyclic AMP Concentrations following an Intravenous Lethal Dose of Cholera Enterotoxin in Rabbits,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Personal Author(s):

Report Date:

1981-01-13

Pagination or Media Count:

14.0

Abstract:

The purpose of this study was to test the hypothesis that a generalized increase in tissue cAMP concentrations after an IV injection of cholera enterotoxin may play a role in the development of biochemical and pathological changes leading to death. Dutch rabbits allocated as control n equals 9 and challenged n equals 13 groups were injected IV with highly purified cholera enterotoxin at a dose of 100 or 200 microgramskg. When death appeared imminent 20-30 hr after inoculation, rabbits were anesthetized plasma urine and 13 different tissue samples were obtained for cAMP determinations. When results from toxin-injection rabbits 100 microgramskg were compared with those of controls, no significant changes were observed in any tissue studied. A higher dose of enterotoxin 200 microgramskg produced significant increases in cAMP concentrations in lung, spinal cord, skeletal muscle, renal cortex, renal medulla and liver. The elevation of cAMP values in selected tissues suggests differences in tissue sensitivity to cholera enterotoxin the data fail to support in toto the proposed hypothesis. Author

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE