Identification of Leukocyte Subpopulations Responsible for the Production of Osteoclast Activating Factor and their Role in Bone Resorption.
Final rept. Oct 77-Sep 80,
ARMY INST OF DENTAL RESEARCH WASHINGTON DC
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Osteoclast Activating Factor OAF was predictably produced in large volume mitogen stimulated MNC cultures. OAF production was independent of DNA synthesis. OAF was produced in mixed leukocyte cultures containing heat inactivated fetal calf serum. OAF production was independent of blastogenesis. OAF activity was isolated in the molecular weight range of 12,000 to 25,000 daltons by S-200 Sephacryl chromatography and high performance liquid chromatography HPLC. OAF activity was concentrated across membranes and by ammonium sulfate precipitation. Subsequent HPLC produced two peaks of bone resorbing activity a major peak at 18,000 daltons and a minor peak at 9,000 daltons. MNC culture supernatants also contained high concentrations of low molecular weight prostaglandins which also are believed to be osteolytic in vitro. Author
- Medicine and Medical Research