Accession Number:

ADA093374

Title:

A Ratio Method for Fluorescence Spectral Deconvolution.

Descriptive Note:

Interim technical rept.,

Corporate Author:

TEXAS A AND M UNIV COLLEGE STATION DEPT OF CHEMISTRY

Personal Author(s):

Report Date:

1980-11-20

Pagination or Media Count:

45.0

Abstract:

A procedure is described for the spectral deconvolution of multicomponent fluorescence data using a ratio method. The only criteria for deconvolution is that each component must have a spectral region where it is the only emitter and that the acquired data sets have a reasonable signal to noise ratio. The ratio method described is an extension of an algorithm previously developed for deconvolution of one-dimensional infrared data. Examples of applications of this algorithm for one-dimensional and two-dimensional fluorescence data are provided. In addition, an example is given as to how fluorescence quenching can be used to aid in spectral deconvolution. Author

Subject Categories:

  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE