Kit Tests for Rapid Detection of Viable Bacteria and Viruses.
Final rept. Jun 79-Jun 80,
GEOMET TECHNOLOGIES INC GAITHERSBURG MD
Pagination or Media Count:
Metabolism-linked reagents for reduction of p-iodonitrotetrazoliumINT were optimized and reaction conditions for detecting viable bacteria on the surface of a filter were defined. The test detected 10 to the 4th power - 10 to the 5th power viable bacteria within 30 minutes. The feasibility of detecting nucleic acids and bacteriophage by binding N-Methylphenazinium methosulfate PMS to the nucleic acid followed by catalytic reduction of nitroblue tetrazoliumWBT was demonstrated. The sensitivity of the test was 10 to the minus 9th power gm of RNA or DNA, corresponding to 10 to the 7th power - 10 to the 8th power virions.
- Chemical, Biological and Radiological Warfare