Accession Number:

ADA090880

Title:

Biochemical Characterization of Rift Valley Fever Virus

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

• Rice, Robert M.
• Erlick, Barry J.
• Rosato, Robert R.
• Eddy, Gerald A.
• Mohanty, Sashi B.

Report Date:

1980-01-01

Pagination or Media Count:

5.0

Abstract:

Rift Valley fever virus RVFV polypeptides were shown to share similar biochemical properties with members of the family Bunyaviradae. Electrophoretic analysis of RVFV revealed one nonglycosylated and two glycosylated major proteins with molecular weights of 25,000, 56,000, and 65,000 respectively. In addiction, a 100,000 MW glycoprotein was found. The 25,000 MW protein was identified as the major nucleocapsid protein. The virion density in CsCl was 1.21 gmi, while that of the nucleocapsid was 1.29 gml. The number and molecular weights of major structural polypeptides of several diverse RVFV isolates were identical. The presence of three RNA segments, large, medium, and small, with molecular weights of 2.7, 1.7, and 0.6 x 10 expn 6 was demonstrated. The close relationships of RVFV proteins and RNA with reported molecular weights of some members of the Phlebotomus fever PHL group viruses were compatible with the serological cross-reactivity among these viruses. These findings support the classification of RVFV with the PHL group viruses in the family Bunyaviradae.

Descriptors:

• *RIFT VALLEY FEVER VIRUS
• BIOCHEMISTRY
• CLASSIFICATION
• ELECTROPHORESIS
• IDENTIFICATION
• PEPTIDES
• POLYMERS
• REPRINTS

Subject Categories:

• Medicine and Medical Research
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE