Studies on PGBX: Fractionation by a Combination of Dialysis and Molecular Exclusion Chromatography.
NAVAL AIR DEVELOPMENT CENTER WARMINSTER PA AIRCRAFT AND CREW SYSTEMS TECHNOLOGY DIRECTORATE
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By combining the techniques of dialysis and molecular exclusion chromatography MEC, a Type III prostaglandin PGBX preparation could be separated into fractions containing different polymer composition and different amounts of in vitro PGBX activity. An over-all purification of PGBX activity was obtained in fraction 3 that contained 20 of the total weight and 40 of the total PGBX activity with a 2 fold purification of specific activity of the PGBX inhibitor factor. This fraction, which was retained by the membrane on aqueous dialysis, and not retained by the membrane on methanol dialysis, had a long retention time on MEC, and gave molecular weight vapor pressure osmometry of 1789. Furthermore this fraction was homogeneous by analytical MEC. Author
- Polymer Chemistry
- Atomic and Molecular Physics and Spectroscopy