Mammalian Toxicological Evaluation of TNT Wastewaters. Volume II. Acute and Subacute Mammalian Toxicity of TNT and LAP Mixture
Final rept. Apr 76-Sep 78,
SRI INTERNATIONAL MENLO PARK CA
Pagination or Media Count:
The acute toxicity of TNT, RDX, LAP 1.6 TNT1.0 RDX, andor LAPI photolyzed LAP 0.032 TNT1.0 RDX, 10 undegraded RDX was determined in mammalian species. In male and female rats, respectively, the acute oral LD50s were TNT, 1320 and 794 mgkg RDX, 71 and about 70 mgkg and LAP, 574 and 594 mgkg or lower, depending on particle size. In male and female mice, respectively, the LD50s were TNT, 660 mgkg both sexes RDX, 75 and 86 mg kg and LAP947 and 1131 mgkg. LAPI was examined in mice only the acute oral LD50s in males and females were 585 and 684 mgkg, respectively. LAP and LAPI produced conjunctivitis, iritis, andor corneal opacity in rabbit eyes the irritation was not totally reversed in unwashed eyes after 7 days and longer. In in vitro microbial assays using microsomal activation Ames Test, TNT was mutagenic. LAP was also mutagenic, and photolysis increased its mutagenicity. In contrast, in vivo cytogenetics studies on rat bone marrow extracts failed to detect an effect of either TNT or LAP on somatic cells. The effects of repeated oral administration of TNT and of LAP were determined in 90-day studies in dogs, rats, and mice. Observations common to the three species treated with either test material were depressed body weight andor weight gain and food intake, mild to moderate hemolytic anemia, enlarged spleens.