GEORGETOWN UNIV WASHINGTON DC LOMBARDI CANCER CENTER
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Major qualitative and quantitative improvements have been made in HLA-D typing. These improvements include advances in homozygous typing cell typing, primed lymphocyte typing and the utilization of these reagents and methods in parallel test systems. First the efficiency of performing and analyzing homozygous typing cell HTC experiments has been increased. Second, additional homozygous typing cells of both known specificity and previously undefined type have been obtained. The selection of this laboratory as one of fourteen major HLA-D typing laboratories by the Eighth International Histocompatibility Workshop demonstrates our current international recognition and will assure our continued leadership in this area. Several important developments in primed lymphocyte typing PLT have been made. Finally the techniques and reagents developed have led to advances in parallel research areas. Homozygous typing cells and HLA-D typed panel were used to determine the association of certain HLA specificities and level of peripheral blood cytotoxic lymphocytes. The primed lymphocytes technique has been used to identify a new potential role for interferon in clinical transplantation. Homozygous typing cells have been transformed into B-cell lines for use as standard reagents in the identification of B-cell antisera.
- Medicine and Medical Research