Analysis of Biologic Samples for Morphine and Morphine-Related Compounds by Gas Chromatographic-Mass Spectrometric Methods
BAYLOR COLL OF MEDICINE HOUSTON TX
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Methods were investigated for the analysis of biologic samples containing morphine and morphine-related compounds through use of bioanalytical systems involving gas chromatograph-mass spectrometer-computer combined instruments. Most of the work was carried out with a quadrupole mass spectrometer designed for chemical ionization work. Methane was used as the reagent gas. The studies included the synthesis of stable isotope labeled compounds and derivatives of morphine and morphine-related compounds, and the development of analytical procedures for the determination of free and total morphine and morphine-related compounds in biologic samples. Mass spectral studies were carried out by electron impact ionization, chemical ionization 0. 5-1 Torr and atmospheric pressure ionization mass spectrometry. The procedures were applied in the analysis of a large number of urinary and blood serum, plasma samples. Methods based upon gas chromatograph-mass spectrometer-computer bioanalytical systems show high specificity and high sensitivity in detection, and are generally regarded as reference methods of analysis. The general procedures developed in the course of this work can be used in other applications.