Effect of Chemical Mutagens on Herpes Virus Transformation and Backmutation of THO Cells.
Final rept. 1 May 78-30 Apr 79,
BRIGHAM YOUNG UNIV PROVO UTAH DEPT OF MICROBIOLOGY
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Quantitative transformation assays detecting morphological transformation of Swiss mouse 3T3 cells by herpes simplex virus type 2 were employed to detect transformation enhancement by potentially mutagenic and carcinogenic chemicals. Hydrazine and 1,2-dimethylhydrazine enhanced the number of transformed foci from two to two and one-half fold in cultures that were exposed to both UV-irradiated virus and chemicals as compared to cultures which were exposed only to the irradiated virus. Other chemicals tested, that did not enhance transformation, were ethylmethanesulfonate, 3-methycholanthrene, 1,1-dimethylhydrazine monomethylhydrazine, JP-5, JP-10, RJ-4, and RJ-5. Mutagenesis tests in THO cells, employing the nonactivated compounds, detected slight mutagenic activity of ethylmethanesulfonate but failed to detect mutagenic activity with any of the other other test compounds. Author