Accession Number:

ADA070914

Title:

Effect of Chemical Mutagens on Herpes Virus Transformation and Backmutation of THO Cells.

Descriptive Note:

Final rept. 1 May 78-30 Apr 79,

Corporate Author:

BRIGHAM YOUNG UNIV PROVO UTAH DEPT OF MICROBIOLOGY

Personal Author(s):

Report Date:

1979-06-01

Pagination or Media Count:

25.0

Abstract:

Quantitative transformation assays detecting morphological transformation of Swiss mouse 3T3 cells by herpes simplex virus type 2 were employed to detect transformation enhancement by potentially mutagenic and carcinogenic chemicals. Hydrazine and 1,2-dimethylhydrazine enhanced the number of transformed foci from two to two and one-half fold in cultures that were exposed to both UV-irradiated virus and chemicals as compared to cultures which were exposed only to the irradiated virus. Other chemicals tested, that did not enhance transformation, were ethylmethanesulfonate, 3-methycholanthrene, 1,1-dimethylhydrazine monomethylhydrazine, JP-5, JP-10, RJ-4, and RJ-5. Mutagenesis tests in THO cells, employing the nonactivated compounds, detected slight mutagenic activity of ethylmethanesulfonate but failed to detect mutagenic activity with any of the other other test compounds. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE