Purification and Mode of Action of a Subcellular Fraction from a Salmonella-Derived Ribosomal Vaccine.
Final rept. 1 Jan 74-31 Aug 75,
TEXAS UNIV AT AUSTIN DEPT OF MICROBIOLOGY
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This report discusses the evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium. Ribosomes isolated from Salmonella typhimurium and the RNA derived from them are highly immunogenic against homologous challenge. Ribosomes washed repeatedly in 1 M NH4Cl lost prophylactic potency and yielded poorly protective RNA. The high salt wash was immunogenic as were the RNA and the protein isolated from the wash. No intrinsic component of ribosomes was removed by the NH4Cl wash, since the ability of both crude and clean ribosomes to function equally well in an in vitro protein synthesizing system was demonstrated. A component with toxic properties similar to those of endotoxin was found in active vaccines but not in weak ones.