Cultivation of Hepatitis Virus in Tissue Culture.
Technical rept. 1 Mar 78-28 Feb 79,
CALIFORNIA UNIV LOS ANGELES DEPT OF MEDICINE
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We have developed tissue culture systems utilizing human hepatocytes and others composed of small bowel cells for the eventual cultivation of hepatitis viruses. Recently we have been successful in developing reagents likely to contain infectious virus. Thus, stool specimens from hepatitis A patients have been obtained and purified. Serum speciments from hepatitis B patients have been obtained and are known to contain whole virus particles. Through studies of posttransfusion hepatitis, infectious plasma known to produce non A non B hepatitis has been identified as has convalescent sera from such patients. Tissue culture studies have been undertaken for the cultivation of hepatitis viruses. Evidence of cytopathic change has not been seen with any of the viral isolates of any of the viral material. However, with hepatitis A, a possible interfering agent has been suggested but definitive evidence in this regard is not available. Work is continuing aimed at further efforts at defining virus host cell interactions in order to stimulate intracellular viral replications.
- Medicine and Medical Research