Development of a Methodology for the Rapid Detection of Coliform Bacteria.
Final rept. 30 Sep 77-30 May 78,
BIORESEARCH INC FARMINGDALE NY
Pagination or Media Count:
A technique is described for the detection and quantification of coliform bacteria in less than 1-12 hours. The detection method is based on the presence of beta-D-galactosidase activity within this class of bacteria. The biochemical reactions exploited in the BioResearch rapid detection method are 1 induction of beta-galactosidase within E. coli by the inducer isopropyl thio beta-D-galactopyranoside IPTG, 2 transport of the fluorescein-conjugated substrate fluorescein di beta-D-galactopyranoside FDG into the bacterial cells and 3 hydrolysis of the substrate to liberate the fluorescent dye fluorescein. This is followed by the detection of the fluorescein dye, whose absorption and fluorescence spactra are well known. Author