Accession Number:

ADA065576

Title:

Development of a Methodology for the Rapid Detection of Coliform Bacteria.

Descriptive Note:

Final rept. 30 Sep 77-30 May 78,

Corporate Author:

BIORESEARCH INC FARMINGDALE NY

Personal Author(s):

Report Date:

1978-05-01

Pagination or Media Count:

40.0

Abstract:

A technique is described for the detection and quantification of coliform bacteria in less than 1-12 hours. The detection method is based on the presence of beta-D-galactosidase activity within this class of bacteria. The biochemical reactions exploited in the BioResearch rapid detection method are 1 induction of beta-galactosidase within E. coli by the inducer isopropyl thio beta-D-galactopyranoside IPTG, 2 transport of the fluorescein-conjugated substrate fluorescein di beta-D-galactopyranoside FDG into the bacterial cells and 3 hydrolysis of the substrate to liberate the fluorescent dye fluorescein. This is followed by the detection of the fluorescein dye, whose absorption and fluorescence spactra are well known. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE