Contributions of Membrane Components to Intracellular Water Order: Research on the Red Blood Cell and Spinach Thylakoid Membranes.
Annual rept. no. 2, 1 Oct 77-30 Sep 78,
WAYNE STATE UNIV DETROIT MICH DEPT OF BIOLOGY
Pagination or Media Count:
Detailed descriptions of the measuresments of the motion of the spin label TEMPAMINE 2,2,6,6-tetramethyl piperidine-N-oxyl-4-amine in spinach thylakoids and red blood cells and ghosts are made. In addition, the possibility of the binding of this spin label to membrane proteins is dealt with. Our evidence shows that TEMPAMINE does not bind detectably to any membrane component or to hemoglobin. Thus, the motions associated with this spin label in the internal aqueous regions of cells arises from the interaction of the spin label with its immediate micro environment and is not due to artifactual binding of this lavel with membrane proteins. Materials and methods are described in detail.