Genetic and Molecular Studies of the Phlebotomus Fever Group of Viruses.
Rept. no. 1 (Annual) 1 Feb-31 Aug 78,
ALABAMA UNIV IN BIRMINGHAM
Pagination or Media Count:
The reporting period represents 6 months since the inception of this project. During this time we have directed our efforts in two main directions which are 1 the selection of viruses and temperature limits for genetic recombination analyses, and 2 characterization of the major elements of selected viruses at the molecular level. With regard to the first direction, for all the viruses we have on hand, we are investigating the temperature ranges for plaque assays and optimal plating conditions. These studies are still in progress. With regard to the second direction, with Karimabad, Punta Toro, Chagres and the Sicilian sandfly fever virus, we have characterized to some extent their genomes and virion proteins. All four viruses have tripartite RNA genomes. For Karamabad and Punta Toro viruses, we have shown that their three viral RNA species large, L, medium, M, and small, S have unique oligonucleotide fingerprints. All four viruses have two or three major viral polypeptides, one or two of which are glycoproteins G1 and G2, while the third is not glycosyated. Electron microscopic evidence indicates that the virus particles are spherical and enveloped with an outer layer of surface spikes.