Comparison of the Response to 'Streptococcus Pneumoniae', 'Salmonella Typhimurium', and Endotoxin in Rat Hepatic RNA Production and Distribution.
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD
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Recently a technique has been devised for quantitative subfractionation of rat liver. This procedure has been used to study the effects of several infectious organisms on hepatic RNA production and distribution, in order to better understand the alterations in hepatic protein production in response to infection. Results from these and previous studies demonstrate an increase in the rate of RNA production in response to Streptococcus pneumoniae infection which reaches significant levels by 10 h and continues to rise through 16 h after infection. Redistribution of RNA into the bound ribosomal fraction takes place at the expense of the free ribosomes during the peak hours of the RNA response. However, in studies using Salmonella typhimurium and its endotoxin, more involvement of the free ribosome fraction during the early stages of the infection is apparent. These data suggest that the hepatic RNA response takes place in two stages, an early endotoxin response, resulting in redistribution of cytoplasmic RNA into free ribosomes, and a later infection response, involving the mobilization of the bound ribosomes. Author