Alterations in Insulin Action by Endotoxin 'in vitro'.
LOUISIANA STATE UNIV MEDICAL CENTER NEW ORLEANS DEPT OF PHYSIOLOGY
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Fat cells isolated from epididymal fat pads of Sprague-Dawley rats were exposed to E.coli endotoxin in vitro and after washing the ensuing alterations in glucose oxidation and antilipolysis studied. Although endotoxin 500 microgramml exhibited an insulin-like effect on basal glucose oxidation, it diminished insulin stimulation of glucose oxidation, seemingly inducing an insulin resistant state. On the other hand, identical doses of insulin elicited significantly greater antilpolytic effects in endotoxin treated, than in untreated cells. Thus, endotoxin exposure in a range of 0.05-200 microgram0.5-ml. cell suspension rendered adipocytes more sensitive to the antilipolytic effect of insulin. Results of this study indicate that the different physiologic actions of insulin on adipocytes are not affected in a uniform mannery by endotoxin exposure in vitro. Author