Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.
Annual progress rept. 1 Aug 77-1 Apr 78,
YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE
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Seven different cell cultures have been tested to a variable extent as substrates for replication of a number of arboviruses and arenaviruses in order to determine the optimal combinations of cells and viruses for preparing microscope slides to use as antigen in the indirect immunofluorescence IF test. Among the viruses were lymphocytic choriomeningitis LCM, Congo-Crimean hemorrhagic fever CCHF, dengue type 1, Japanese encephalitis JE, eastern EEE and Venezuelan VEE equine encephalitis and Junin. A basic procedure for the preparation of slides each with 12 samples of a given virus--spot-slides--has been developed. Studies have been undertaken to determine how soon after infection of experimental animals will antibodies be detectable by IF test as compared with other serological tests, complement fixation CF, hemagglutination inhibition HI and neutralization. The models of infection employed were, Langat virus in hamsters and JE in rabbits and mice. Spot-slides and the IF test were used for detection of antibodies against Lassa fever, CCHF and Pichinde viruses in human sera. The results compared favorably with those obtained by CF and, when applicable, HI. Author
- Medicine and Medical Research