Radiometric Methods for Rapid Diagnosis of Viral Infection.
Rept. no. 4 (Final). 1 Feb 75-31 Jan 78,
JOHNS HOPKINS UNIV BALTIMORE MD
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Three radiometric techniques were investigated for monitoring the effects of herpes simplex virus type I and II and cytomegalovirus on the metabolism of human embryonic lung fibroblast WI-38 monolayers. The study was based on the hypothesis that 1 early metabolic effects of virus on the cell culture can be used as an indication for the presence of virus 2 the specificity can be achieved by neutrolization of virus effects with specific antiserum and 3 radiometric technique can be used to measure these metabolic effects of virus. Glucose oxidation by infected and uninfected cells was measured by the output of C-14 CO2 using Bactec DNA synthesis was monitored by H-3 thymidine incorporation measured by liquid scintillation counting or by I-125 iododeoxyuridine incorporation measured non-destructively by gamma scintillation counting. Radiometric results were compared to those obtained from visual examination for cytopathic effects in the same cell line. Herpes simplex virus type I inhibited glucose oxidation by WI-38 cells. Herpes simplex virus type I and II, and cytomegalovirus stimulated DNA synthesis by WI-38 cells. These metabolic effects were observed before any signs of cytopathic effects and could be neutrolized with antiserum. These radiometric techniques for detection of viral effect on cellular metabolism are simple, objective and quantitative. Author
- Medicine and Medical Research