Accession Number:

ADA056539

Title:

Exogenous Nucleotide Protection of Mammalian Cells from Diphtheria Toxin.

Descriptive Note:

Interim rept.,

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Personal Author(s):

• Middlebrook,John L.
• Dorland,Rebecca B.

Report Date:

1978-06-14

Pagination or Media Count:

25.0

Abstract:

Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base-specific and phosphate chain-length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by nucleotide-induced phosphorylation. Nucleotides antagonized the binding of diphtheria toxin to its cell surface receptor in a manner that correlated with the degree of protection. It was concluded that cellular protection from diphtheria toxin by nucleotides results from inhibition of toxin-receptor binding and that nucleotides therefore may serve as valuable research tools for future studies. Author

Descriptors:

• *NUCLEOTIDES
• MAMMALS
• CELLS(BIOLOGY)
• TOXINS AND ANTITOXINS

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE