Investigation of Immunoregulatory Alphaglobulin (IRA) in Shock and Trauma.
Annual progress rept. 1 Jul 77-30 Jun 78,
PETER BENT BRIGHAM HOSPITAL BOSTON MASS
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The major accomplishment of the past year of research was the demonstration that energy in patients following major trauma and burns, as revealed in vivo by lack of delayed hypersensitivity responsiveness, was accompanied by and correlated with high levels of immunosuppressive activity in the patients serum. We have also demonstrated that the ability of peripheral blood lymphocytes from trauma patients to form T-cell rosettes in vitro is associated with high levels of circulating immunosuppressive activity in the serum. This association is further borne out by the fact that patients whose lymphocytes form diminished numbers of T-cell rosettes, as compared with control individuals frequently show markedly improved rosette forming ability after multiple in vitro washings of their lymphocytes. We have begun to characterize the suppressive material washed from the lymphocytes surface and have defined an active fraction by gel exclusion chromatography. We have also determined that while the peripheral blood lymphocytes of patients following trauma and burns frequently demonstrate diminished responsiveness to stimulation by phytohemagglutinin PHA in tissue culture, diminished PHA responsiveness is not correlated with the presence or absence of circulating immunosuppressive polypeptide material in the serum.
- Anatomy and Physiology