Role of Cellular Components of Mosquito Cells in Viral Replication and Transmission.
Annual summary rept. 1 May 77-20 Feb 78,
INDIANA UNIV AT INDIANAPOLIS SCHOOL OF MEDICINE
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Two mosquito cell proteins have been identified by polyacrylamide gel electrophoresis as being associated with banzivirus ggrown in mosquito cell. This has been demonstrated in a number of ways. First, prelabeled cellular components are incorporated in the virus. Second, the two mosquito cell proteins are present in viruses purified by at least 180 fold. Third, exogeneously added cellular proteins when added to the virus prior to purification are not detected in the purified virus. Fourth, reaction of anti-mosquito sera with purified banzivirus grown in mosquito cells has been detected in immunodiffusion assays. Both mosquito cell proteins appear to be glycoproteins in that they contain label derived from 3H-glucosamine. Immunization of mice with uninfected mosquito cells protects against infection by banzivirus grown mosquito cells. Thus protection is not due to any media components and is due, in part, to the homoral response of the mouse as shown by the fact that antisera to uninfected mosquito cells neutralizes infectivity of mosquito cell-grown banzivirus. Author
- Medicine and Medical Research