Accession Number:

ADA055661

Title:

Structure-Function and Immunochemical Studies on Cobrotoxin.

Descriptive Note:

Final rept. Apr 77-Mar 78,

Corporate Author:

NATIONAL TSING HUA UNIV HSINCHU (TAIWAN) INST OF MOLECULAR BIOLOGY

Personal Author(s):

Report Date:

1978-05-01

Pagination or Media Count:

32.0

Abstract:

The heterogeneity of precipitating and non-precipitating antibodies to cobrotoxin was demonstrated by their elution pattern on cobrotoxin-Sepharose and chromatography on DEAE-cellulose column. Gel filtration patterns on Sepharose 6B column revealed that the soluble complexes from non-precipitating antibody and HNB-formed cobrotoxin at a different molar ratio are emerged in the void volume, indicating that the molecular weight of the soluble complex is around 4,000,000 or larger. Unreacted free non-precipitating antibody coincided with the peak of IgG and was proved to be free from HNB-cobrotoxin. The molar ratio of antibody to antigen for the soluble complex was found to be 0.79 to 0.97 indicating that 1.58 to 1.94 molecules of non-precipitating antibody bound to HNB-cobrotoxin instead of three molecules in the case of precipitating antibody. Author

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE