Structure-Function and Immunochemical Studies on Cobrotoxin.
Final rept. Apr 77-Mar 78,
NATIONAL TSING HUA UNIV HSINCHU (TAIWAN) INST OF MOLECULAR BIOLOGY
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The heterogeneity of precipitating and non-precipitating antibodies to cobrotoxin was demonstrated by their elution pattern on cobrotoxin-Sepharose and chromatography on DEAE-cellulose column. Gel filtration patterns on Sepharose 6B column revealed that the soluble complexes from non-precipitating antibody and HNB-formed cobrotoxin at a different molar ratio are emerged in the void volume, indicating that the molecular weight of the soluble complex is around 4,000,000 or larger. Unreacted free non-precipitating antibody coincided with the peak of IgG and was proved to be free from HNB-cobrotoxin. The molar ratio of antibody to antigen for the soluble complex was found to be 0.79 to 0.97 indicating that 1.58 to 1.94 molecules of non-precipitating antibody bound to HNB-cobrotoxin instead of three molecules in the case of precipitating antibody. Author