Common Blood Bank Contaminants Evaluated by the Bactec Radiometric System.
Rept. for Feb 76-Jun 77,
LETTERMAN ARMY INST OF RESEARCH SAN FRANCISCO CALIF
Pagination or Media Count:
Research techniques in blood preservation require frequent entry into blood bags stored at 4 C. Rapid detection of possible contamination and careful evaluation of a selected bacteriological method are essential. BactecRegistered radiometric methods Johnston Laboratories, Inc., Cockeysville, MD were evaluated. Data were collected concerning detection of small numbers of organisms 1 to 1000 colony forming units per milliliter. Sudies were done with 0.5 to 3 mlvial inoculum and at temperatures of 35, 22-24, and 4 C. The evidence indicates that the Bactec radiometric procedure is adaptable to smaller samples and to different incubation temperatures than recommended by Bactec for non-fastidious organisms only. Oxidative psychrophilic and other fastidious organisms were detected more frequently and faster using subculture and gram stain methods. Author
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