Accession Number:

ADA051582

Title:

Detection of Explosive Vapors by Enzymatic Methods -- Laboratory Validation and Systems Analysis.

Descriptive Note:

Final rept. Oct 76-Nov 77,

Corporate Author:

BECKMAN INSTRUMENTS INC ANAHEIM CALIF ADVANCED TECHNOLOGY OPERATIONS

Report Date:

1978-01-01

Pagination or Media Count:

31.0

Abstract:

The biological approach to mine detection selected for development in 1973 is based on two sequential chemical reactions. In the first stage, TNT reacts with NADH in a reaction which is catalyzed by the specific enzyme TNT reductase, or TNTase. In the second stage, residual NADH is used in another enzyme catalyzed reaction to reduce FMN to FMNH2. The FMNH2 further reacts with dissolved oxygen and an aldehyde tetradecanal to produce photons having a peak at a wavelength of 492 nm and other reaction products. It is this final reaction that provides the very high sensitivity of the method. It is conducted in the field of view of a PMT, which is capable of sensing the loss of ten to the 9th power or less molecules of NADH due to the first-stage TNT reaction.

Subject Categories:

  • Biochemistry
  • Test Facilities, Equipment and Methods
  • Ammunition and Explosives

Distribution Statement:

APPROVED FOR PUBLIC RELEASE