Hypothermia Following Injection of 2-Deoxy-D-Glucose into Selected Hypothalamic Sites,
ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MASS
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From previous studies with 2-deoxy-D-glucose 2-DG an inhibitor of glucose utilization, we postulated that the resultant intracellular glucopenia affects central neuronal pathways involved in the control of peripheral heat production. In this investigation we have delineated these thermoregulatory sites by stereotaxically injecting micro-quantities of 2-DG into the hypothalamus of the rat and monitoring core temperature T-re. After stabilization of T-re, 2 microliters of 20 micrograms of 2-DG was injected into 350-400 g rats at 23 or - 1 C. Significant decreases in T-re were noted for the anterior hypothalamic, ventromedial and dorsomedial nuclei as well as the lateral and posterior hypothalamic areas. With the ventral premammillary nucleus PMV mean nadir T-re decreases of -1.1 C occurred 1 hour after administration of 2-DG, was significantly depressed after 3.12 hours, and returned to basal values after 4 hours. Dose dependant response was observed only for this nucleus. Of a total of 21 sites studied in both the anterior and posterior hypothalamus, the PMV, an area of unknown physiological function, was the most sensitive to glucose deprivation.
- Anatomy and Physiology