Genetics of Novel Hybrid Bacteriophage and Development of Generalized Transducing System for Salmonella typhosa.
Annual progress rept. no. 4, 1 Jan-31 Dec 77,
TEMPLE UNIV PHILADELPHIA PA SCHOOL OF MEDICINE
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Various hybrid phages between Salmonella Phage P22 and coliphages such as lambda and phi-80 have been isolated by using their common bacterial hosts, E. coli-S. typhimurium hybrids. Among those hybrid phages, lambda-P22dis and phi-80-P22 hybrid classes carry a large genetic segment of P22 phage containing the Im gene in addition to the c genes. Since the Salmonella somatic antigen conversion gene al and tail component gene 9 of P22 are located between the c and Im genes, these hybrid phages should carry the al and tail gene 9 of P22. Therefore we studied the antigen conversion of various bacterial hosts and the P22 tail gene expression, although these genes are dispensable for these hybrid phages. Both lambda-P22dis and phi-80-P22 can convert E. coli-S. typhimurium with somatic antigen 0-1. However, E. coli K12 was not converted by these hybrid phage classes. This can be explained by the fact that E. coli does not have 0-1 antigen acceptor sites which are gal-rha-man repeating units of Salmonella typhimurium cell surface. Moreover, the P22 tail component gene 9 in lambda-P22dis is expressed during lytic replication of lambda-P22dis hybrid phage. Author