Purification of Alveolar Epithelial Type II Cells from Rat Lung.
Final rept. Jan 75-Jan 77,
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
Pagination or Media Count:
Prolonged inhalation of above-ambient concentrations of oxygen can lead to severe damage of the lung and to the compromise of respiratory function. Several observations indicate that the surface properties of the material adsorbed at the alveolar air-liquid interface may be affected by such exposures. It is not clear, however, whether these perturbations result from the direct damage to the cells metabolizing surfactant, or whether they occur from damage to other lung cells resulting in an effusion of interfering materials into the alveoli and to alveolar edema. To help differentiate between these alternatives the author reasoned that methods must be used by which the metabolism of pulmonary surfactant can be studied directly. These include 1 Isolation of certain proteins associated with this material 2 Purification of enriched populations of cells responsible for the synthesis of this material 3 Quantification of the content of surfactant apoprotein in these cells and 4 Investigation of some of the metabolic activities of these cells, particularly with regard to the metabolism of the proteins of surfactant. He has developed the necessary techniques and provides detailed results of his investigations in this report.