Mechanism of Action of Antigens.
Annual progress rept.(Final),
PITTSBURGH UNIV PA DEPT OF PATHOLOGY
Pagination or Media Count:
A major advance of this research project has been the quantitation of IgG and IgM immunoglobulins present in the plasma membranes of thymic lymphocytes which have been considered until now to lack membrane immunoglobulin. The import of this finding is that the evidence for thymic lymphocytes as antigen-reactive cells is consistent with the postulate of immunoglobulin as cell receptor for antigen. Thymic lymphocyte membranes are not as efficiently labelled as membranes from splenic lymphocytes and more specifically, IgG immunoglobulin in thymic lymphocytes is not labelled at all. The investigator concludes that IgG immunoglobulins are buried within the matrix of the plasma membrane and not accessible to external labelling reagents. He has also selectively extracted thymic lymphocyte glycoproteins using the lithium diiodosalicylate method. The delineation of differences between high responder and low responder lymphotocytes is now being studied by in vitro biosynthesis of lymphocyte membrane components using incorporation of radioactive amino acids.
- Anatomy and Physiology