In vitro Effects of Methylprednisolone Sodium Succinate and E. coli Organisms on Neutrophils in Baboon Blood.
OKLAHOMA UNIV HEALTH SCIENCES CENTER OKLAHOMA CITY
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The corticosteroid, methylprednisolone sodium succinate MP, has been observed to prevent hypoglycemia in experimental septic shock however, detrimental actions of various corticosteroids on polymorphonuclear leukocyte function have been reported. The present study was designed to determine if MP depresses glucose metabolism of leukocytes or adversely affects neutrophil survival, or whether it modifies the mortality rate of live E. coli in baboon blood in vitro. Results show that therapeutically effective concentrations 13 microgramml blood and high doses 130 microgramml blood of MP exert no detrimental influences on glucose utilization or survival of neutrophils in the absence or presence of E. coli organisms in concentrations of 4.2x 10 to the 7th power and 2.3x 10 to the 8th power organismsml blood. E. coli organisms, however, increase neutrophil mortality rate and glucose uptake of the blood. These findings support the view that MP does not adversely influence leukocyte metabolism and survival, nor does it modify the mortality rate of live E. coli. Author
- Anatomy and Physiology